Spectrophotometric measurement of NAG (N-Acetyl-β-D-glucosaminidase) enzyme activity.
This kit procedure incorporates the VRA-GlcNAc substrate when a spectrophotometric measurement is required.
Figure 1. VRA-glcNAc substrate
| Description | Product Code |
| 50 test kit | 1300050 |
| 100 test kit | 1300100 |
Kit Contains: Rapichrome Substrate V, Incubation Buffer R, Colour Development Buffer A
Optional – NAG enzyme Controls (Low, Medium and High)
NAG/Creatinine controls*
| Description | Product Code | MSDS |
| High: NAG 2500-3500 μmol/h/L; creatinine 18 mM | 1300008 | |
| Medium: NAG 1400-1800 μmol/h/L; creatinine 9 mM | 1300009 | |
| Low: NAG 750-950 μmol/h/L; creatinine 3 mM | 1300010 |
* These NAG/creatinine controls are for use with VRA-NAG kits only (1300050/1300100). For use with the MNP-NAG kits (1290050/1290100), please click here.
References: 1. Posci et al., (1990) VRA-GLcNAc: A novel substrate for N-Acetyl-β-D-glucosaminidase applied to the assay of this enzyme in urine. Clin chem 36: 1884-1888.
2. Posci et al (1991) Assay of N-Acetyl-β-D-glucosaminidase in neonate urine: comparison of two new colorimetric methods using MNP-GLcNAc and VRA-GLcNAc as substrates. Ann Clin Chem 29:292-295.
3. Sheira et al (2015) urinary biomarker N-Acetyl-β-D-glucosaminidase can predict severity of renal damage in diabetic nephropathy. J Diabet & Met Dis. 14:4.DOI 10.1186/s402-015-0133-6.
Please enquire for further details and indicate if the assay is to be carried out in serum so that we can supply a modified kit and assay procedure.