Chromogenic substrates for the assay of β-N-acetyl-glucosaminidase (EC 3.2.1.52)

β-N-acetylglucosaminidase is also known as N-acetyl-beta-hexosaminidase, NAG or HEX In mammals, β-N-acetylglucosaminidase (NAG) is a lysosomal enzyme which catalyses the breakdown of gangliosides and other related glycosides. The assay of urinary NAG activity has been used for the diagnosis of kidney damage and diabetic complications. Elevated serum NAG activity is also associated with some cancers. It is more sensitive than LDH in tissue culture.

Enzyme	Substrate	Product Code	MSDS
β-glucosaminidase/β-hexosaminidase	MNP-GlcNAc	1050	MNP-GlcNAc
	VRA-GlcNAc	1051	VRA-GlcNAc
	VLM-GlcNAc	1052	VLM-GlcNAc
	VLPa-GlcNAc	1070	VLPa-GlcNAc

These products are available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request. References: Aamlid et al (1989) Development of improved chromogenic substrates for the detection and assay of hydrolytic enzymes. Chem & In., 106-108. Aamlid et al., (1990) New Colorimetric substrates for the assay of glycosidases. Carbohydrate Research, 205, C5-C9.  Posci et al (1990) VRA-GlcNAc: Novel subsite for N-acetyl-β-D-glucosaminidase applied to the assay of this enzyme in urine. Clin. Chem. 36, 1884-1888.

1050 MNP-GlcNAc powder

This substrate is a pale-yellow powder which on hydrolysis by β-N-acetylglucosaminidase releases a soluble product that is red at pH> 9.5 and is measured at 505 nm. The Extinction coefficient of the released chromogenic product is 27,000 Lmol^-1cm^-1 2-Methoxy-4-(2-nitrovinyl)-phenyl-2-acetamido-2-deoxy-β-D-glucopyranoside: C₁₇H₂₂O₉N₂ Molecular weight: 398 gmol^-1       CAS number: 70622-74-1 MSDS – MNP-GlcNAc

1051 VRA-GlcNAc powder

This substrate is a bright yellow powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is red in colour at pH> 9.5 and can be measured at 492 nm. Extinction coefficient of the released chromogenic product is 37,000 Lmol^-1cm^-1. Ammonium-5-[4-(2-acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxymethylene]-2-thioxothiazolidin-4-one-3-acetate hydrate: C₂₁H₂₇N₃O₁₀S₂      Molecular weight: 546 gmol^-1      CAS number: 125261-87-2 MSDS – VRA-GlcNAc

1052 VLM-GlcNAc powder

This chromogenic substrate is an orange powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 524 nm. Extinction coefficient of the released chromogenic product is 35,000 Lmol^-1cm^-1. 4-{2-[4-(2-Acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-methyl-quinolinium iodide: C₂₇H₃₁N₂O₇.I         Molecular weight: 622 gmol^-1 MSDS – VLM-GlcNAc

1070 VLPa-GlcNAc powder

This chromogenic substrate is an orange powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 525 nm. Extinction coefficient of the released chromogenic product is 35,000 Lmol^-1cm^-1. 4-{2-[4-(2-Acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-propan-3-oate-quinolinium bromide: C₂₉H₃₄N₃O₉.Br       Molecular weight: 648 gmol^-1 MSDS – VLPa-GlcNAc